Inoculated media are examined daily for D.В oviparasitica. Clumps of parasitized eggs were washed in sterile water and added to cornmeal agar (cornmeal infusion, 50В g agar, 15В g water, 1В L), glucose-peptone agar (glucose, 10В g peptone, 10В g agar, 16В g water, 1В L), or YPSS agar (yeast extract, 4В g K2HPO4, 1В g MgSO4вЂ“7H20, 0.5В g soluble starch, 20В g agar, 16В g water, 1В L). Stirling and Mankau (1978a) collected the gelatinous matrix of Meloidogyne egg masses from host plants in the field and partially dissolved by treating in 1% NaOC1 for about 2В min, and eggs were examined for parasitic fungi. By combining the ITS sequence with morphologic characteristics, a new anamorphic species was described and illustrated together with its teleomorph. Conidia were elongated ellipsoids, 1вЂ“2 septate, mostly 1 septate. Conidiophores were either not branched or occasionally branched, bearing divergent sterigmata on the tip with a single conidium on each. (2009) isolated D.В alba, a new species from the ascospores of Orbilia alba collected in Wenshan County, Yunnan Province, China. Therefore, it is described as a new anamorphic species, D.В coccinella. Comparison of its morphologic and biologic characters and analysis of sequence data of the rDNA in ITS region suggest that the fungus is a separate species. Simple conidiophore that bears a single, cylindrical, 1-7- septate conidium with a round distal end, a monoblastic conidiogenous cell, cylindrical, and absence of nematode-trapping capability distinguish this anamorphic fungus from other Dactylella species. coccinella from the teleomorphic fungus Orbilia coccinella and described it as a new anamorphic species.

Sterling and Mankau (1978) isolated D.В oviparasitica from root knot nematode egg masses ( Meloidogyne spp.). Sunitha, in Beneficial Microbes in Agro-Ecology, 2020 3 Isolation of Dactylella The latter formula was superior to any formerly developed formulas and may thus have potential for the improved artificial diet mass rearing of C. septempunctata fed on the diet than on A. The weight gain and fecundity of adults reared on the improved artificial diet were 87.46 and 62.70% of those reared on Aphis craccivora the survival rate and hatch rate were similar between the diet-fed and aphid-fed, while the preoviposition period was significantly shorter for C. A follow-up fed with a selection of improved formulas confirmed the validity of the optimization as predicted by the orthogonal array analysis, indicating the usefulness of this method for selecting artificial diets for C. Results indicated that pork liver, sucrose, yolk, yeast powder, juvenile hormone, pumpkin and honey were the main ingredients of the artificial diet contributing to weight gain and survival rate of adults, preoviposition period, fecundity and hatching rate. The 16 factors were included: pork liver, infant formula, sucrose, olive oil, yolk, corn oil, yeast powder, cholesterol, casein, casein hydrolysate, vitamin powder, 65% juvenile hormone III, protein powder, vitamin E, honey and pumpkin.

The parameters of weight gain and survival rate of adults, preoviposition period, fecundity and hatching rate of diet-fed adults were monitored. In this study, an orthogonal array design with 16 factors at two levels (2 16) was performed to develop an artificial diet rearing the adults of seven spotted ladybird beetle Coccinella septempunctata.

Coccinellidae coccinella